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Apolipoprotein O-Like Antibody

[47945]
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  • 询价
  • Signalway Antibody已认证
  • 47945
  • 2025年09月23日
  • WB, IHC
  • Rabbit
  • Human;Mouse;Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Apolipoprotein O-Like.

    • 形态

      liquid

    • 保存条件

      Store at -20˚C

    • 克隆性

      Polyclonal

    • 适应物种

      Human;Mouse;Rat

    • 保质期

      12 months

    • 抗原来源

      Rabbit

    • 供应商

      南京赛戈巍生物科技有限公司

    • 宿主

      Rabbit

    • 应用范围

      WB, IHC

    • 靶点

      Uniprot:Q6UXV4

    • 抗体英文名

      Apolipoprotein O-Like Antibody

    • 规格

      50ul/100ul

    别名:CXorf33; FAM121A; Apolipoprotein O-like; Protein FAM121A
    计算分子量:30KD
    配方:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    应用详情:

    Western blotting:1:500 - 1:1000


    图片:

    产品细节图片1

    Western blot analysis of Apolipoprotein O-Like expression in Hela (A), H446 (B), mouse kidney (C), mouse heart (D), rat kidney (E), rat heart (F) whole cell lysates.

    ,

    产品细节图片2

    Immunohistochemical analysis of Apolipoprotein O-Like staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


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    图标文献和实验
    相关实验
    • Antibody Conjugation

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • Antibody cleanup

      seven times, or as often as necessary to decrease the background. Pellet cells. Save antibody supernatant, which is now diluted 1:5 from its initial concentration. These antibodies can be stored at 4o C for several weeks or at -70o C for longer periods

    • Antibody Conjugation Protocol

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

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