Beta-NaCH (phospho-Thr615) Ant

别名:Amiloride-sensitive sodium channel subunit beta; Beta-NaCH; Epithelial Na(+) channel subunit beta; Beta-ENaC; ENaCB; Nonvoltage-gated sodium channel 1 subunit beta; SCNEB
计算分子量:73KD
配方:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Western blotting:1:500 - 1:1000
Immunohistochemistry:1:50 - 1:100
Immunofluorescence:1:100 - 1:300
图片:

Western blot analysis of Beta-NaCH (phospho-Thr615) expression in HeLa whole cell lysates.

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Immunohistochemical analysis of Beta-NaCH (phospho-Thr615) staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Immunofluorescent analysis of Beta-NaCH (phospho-Thr615) staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.