- 询价
- gelatins
- JLC163922
- 上海
- 2025年07月16日
- 人/动物/植物
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
多克隆
- 抗体名：
Anti-VPS50 antibody
- 适应物种：
人/动物/植物
- 浓度：
1.32 mg/ml
- 保存条件：
-20°C
- 规格：
25 μl/100 μl/200 μl
Cat. No. JLC163922
Package 25 μl/100 μl/200 μl
Storage -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Product overview
Description Anti-VPS50 rabbit polyclonal antibody
Applications ELISA, IHC
Immunogen Synthetic peptide of human VPS50
Reactivity Human, Mouse, Rat
Content 1.32 mg/ml
Host species Rabbit
Ig class Immunogen-specific rabbit IgG
Purification Antigen affinity purification
Target information
Symbol VPS50
Full name VPS50, EARP/GARPII complex subunit
Synonyms VPS54L; CCDC132
Swissprot Q96JG6
Target Background
Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane. Within the EARP complex, required to tether the complex to recycling endosomes. Not involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).
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文献和实验IMMUNOSCREENING OF WITH ANTIBODY PROBES
in 10mM IPTG (MW=238.31). Briefly blot the excess solution from the filters, and wrap them in cellophane. These should be moist and stored at 4°C. We typically adsorb serum with five 150 mm Petri dishes of lysed E. coli to reduce anti-E. coli antibody
with 6 ml Buffer B, 6 ml Buffer A, and 6 ml Buffer B. Wash with Storage Buffer and hold at 4°C. • To purify the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes. • Wash with 50 ml 10 mM Tris
Antibody Humanization by CDR Grafting
of antibody therapeutics in the 1980s, when the major obstacle was the human anti-murine antibody (HAMA) response (1 , 2 ). The HAMA response occurred in up to 50% of patients upon the administration of murine hybridoma-derived antibodies (3 ) and severely
