品牌:CST,产品名称:Perilipin (D1D8) XP Rabbit mAb #9349 英文名称:Perilipin (D1D8) XP® Rabbit mAb Product name:Perilipin (D1D8) XP® Rabbit mAb #9349 Source:Rabbit Sensitivity:Endogenous MW(kDa):13 Applications:WB , IP , IHC , IF , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues around Ile419 of human perilipin protein. Background: Triacylglycerol is stored in lipid droplets as a primary energy reserve. Perilipin is localized at the periphery of lipid droplets and serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as hormone-sensitive lipases (2). Hence, perilipin plays a pivotal role in lipid storage (2,5).
Perilipin (D1D8) XP® Rabbit mAb detects endogenous levels of total perilipin/perilipin-1 protein.
Species Reactivity:
Human, Mouse
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Immunohistochemistry (Paraffin)
1:100
Immunofluorescence (Frozen)
1:200
Immunofluorescence (Immunocytochemistry)
1:200
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.