品牌:CST,产品名称:Pdx1 (D59H3) XP Rabbit mAb #5679 英文名称:Pdx1 (D59H3) XP® Rabbit mAb Product name:Pdx1 (D59H3) XP® Rabbit mAb #5679 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):36 Applications:WB , IP , IF , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val274 of human Pdx1 protein. Background: The pancreatic duodenal homeobox gene-1 (Pdx1) is a transcription factor that contributes to pancreas development, pancreatic β-cell differentiation, and mature β-cell function (1,2). It plays an essential role in the commitment of endoderm to a pancreatic and later β-cell phenotype (2,3). In the mature pancreas, Pdx1 expression is more restricted to the pancreatic β-cells (3), where it promotes the expression of genes important for β-cell functions such as insulin, glucokinase, and Glut2 (4-6). Mutations of the corresponding Pdx1 gene may be associated with diabetes and cases of pancreatic insufficiency (7).
Pdx1 (D59H3) XP® Rabbit mAb detects endogenous levels of total Pdx1 protein.
Species Reactivity:
Human, Mouse, Rat
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Immunofluorescence (Frozen)
1:400
Immunofluorescence (Immunocytochemistry)
1:400
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.