品牌:CST,产品名称:PDCD4 (D29C6) XP Rabbit mAb #9535 英文名称:PDCD4 (D29C6) XP® Rabbit mAb Product name:PDCD4 (D29C6) XP® Rabbit mAb #9535 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):45-60 Applications:WB , IP , IHC , IF , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human PDCD4 protein. Background: PDCD4 (programmed cell death protein 4) was identified as a suppressor of neoplastic transformation (1). It interacts with eukaryotic initiation factors eIF4AI and eIF4AII (2). PDCD4 colocalizes with eIF4A in the cytoplasm and inhibits the activity of eIF4A as an ATP-dependent RNA helicase (2). PDCD4 inhibits cap-dependent translation (2). Upon stimulation by mitogens, PDCD4 is phosphorylated on Ser67 by S6K1 kinase and then degraded through the ubiquitin pathway leading to enhanced translation and cell growth (3).
PDCD4 (D29C6) XP® Rabbit mAb detects endogenous levels of total PDCD4 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Immunohistochemistry (Paraffin)
1:200
Immunofluorescence (Immunocytochemistry)
1:600
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.