品牌:CST,产品名称:PD-1 (D4W2J) XP Rabbit mAb #86163 英文名称:PD-1 (D4W2J) XP® Rabbit mAb Product name:PD-1 (D4W2J) XP® Rabbit mAb #86163 Source:Rabbit Sensitivity:Endogenous MW(kDa):25 Applications:WB , IP , IHC , F , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala274 of human PD-1 protein. Background: The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T-cells, PD-1 is expressed in activated B-cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).
PD-1 (D4W2J) XP® Rabbit mAb recognizes endogenous levels of total PD-1 protein.
Species Reactivity:
Human
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:200
IHC-Leica® Bond™
1:200
Immunohistochemistry (Paraffin)
1:200
Flow Cytometry
1:200
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.