品牌:CST,产品名称:PCNA (PC10) Mouse mAb #2586 英文名称:PCNA (PC10) Mouse mAb Product name:PCNA (PC10) Mouse mAb #2586 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):36 Applications:WB , IP , IHC , IF , F , Purification: Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein obtained from pC2T. Background: Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation.
PCNA (PC10) Mouse mAb detects endogenous levels of PCNA protein.
Species Reactivity:
Human, Mouse, Rat, Monkey, Bovine, Pig
Application
Dilutions
Western Blotting
1:2000
Immunoprecipitation
1:100
Immunohistochemistry (Paraffin)
1:4000
Immunofluorescence (Immunocytochemistry)
1:2400
Flow Cytometry
1:800
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.