PathScan® Intracellular Signaling Array Kit (Chemiluminescent Readout) detects the indicated cellular proteins and signaling nodes only when phosphorylated or cleaved at the specified residues. (see Array Target Map). No significant cross-reactivity has been observed between targets. This kit is optimized for cell lysates diluted to a total protein concentration between 0.2 and 1 mg/ml (see kit protocol).
Storage:
Kit should be stored at 4°C with the exception of Lysis Buffer, which is stored at –20°C (packaged separately).
Product Includes
Quantity
Cap Color
Array Slides - Intracellular Signaling Array Kit
2 ea
16-Well Gasket
2 ea
Chemiluminescent Development Folder
2
Array Blocking Buffer
5 ml
Red
Detection Ab Cocktail (10X) - Intracellular Signaling Array Kit
300 µl
White
HRP-Linked Streptavidin (10X)
300 µl
Clear
Product Description:
The PathScan® Intracellular Signaling Array Kit (Chemiluminescent Readout) is a slide-based antibody array founded upon the sandwich immunoassay principle. The array kit allows for the simultaneous detection of 18 important and well-characterized signaling molecules when phosphorylated or cleaved. Target-specific capture antibodies have been spotted in duplicate onto nitrocellulose-coated glass slides. Each kit contains two 16-pad slides, allowing the user to test up to 32 samples and generate 576 data points in a single experiment. Cell lysate is incubated on the slide followed by a biotinylated detection antibody cocktail. Streptavidin-conjugated HRP and LumiGLO® Reagent are then used to visualize the bound detection antibody by chemiluminescence. An image of the slide can be captured with either a digital imaging system or standard chemiluminescent film. The image can be analyzed visually or the spot intensities quantified using array analysis software.