品牌:CST,产品名称:p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (SepharoseBead Conjugate) #5736
英文名称:p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Sepharose® Bead Conjugate)
Product name:p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Sepharose® Bead Conjugate) #5736
Isotype:Rabbit IgG
Sensitivity:Endogenous
Applications:IP ,
Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase.
Background: Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.
Entrez-Gene Id
5595, 5594
Swiss-Prot Acc.
P27361, P28482
Specificity / Sensitivity:
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Sepharose® Bead Conjugate) detects endogenous levels of total p44/42 MAPK (Erk1/2) protein. The antibody does not cross-react with JNK/SAPK or p38 MAP kinase. The addition of 0.1% SDS to the immunoprecipitation reaction may improve pulldown efficiency. Under some conditions this conjugate may immunoprecipitate p44/Erk1 preferentially over p42/Erk2.
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish, Bovine, Dog, Pig, C. elegans
Species predicted to react based on 100% sequence homology:
Chicken
Application |
Dilutions |
Immunoprecipitation |
1:20 |
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Product Description:
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Sepharose® Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695.