品牌:CST,产品名称:p38α MAPK (7D6) Rabbit mAb #2371
英文名称:p38α MAPK (7D6) Rabbit mAb
Product name:p38α MAPK (7D6) Rabbit mAb #2371
Source:Rabbit
Sensitivity:Endogenous
MW(kDa):43
Applications:WB ,
Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human p38α MAPK.
Background: p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
Entrez-Gene Id
1432
Swiss-Prot Acc.
Q16539
Specificity / Sensitivity:
p38α MAP Kinase (7D6) Rabbit mAb detects endogenous levels of total p38α MAPK protein. This antibody does not cross-react with other p38 MAPK isoforms such as β, γ or δ.
Species Reactivity:
Human, Mouse, Rat, Monkey
Application |
Dilutions |
Western Blotting |
1:1000 |
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.