品牌:CST,产品名称:p38α MAPK (5F11) Mouse mAb #9217 英文名称:p38α MAPK (5F11) Mouse mAb Product name:p38α MAPK (5F11) Mouse mAb #9217 Source:Rabbit Sensitivity:Endogenous MW(kDa):40 Applications:WB , Purification: Monoclonal antibody is produced by immunizing animals with a recombinant p38 MAPK fusion protein. Background: p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
p38α MAP Kinase (5F11) Mouse mAb detects endogenous levels of p38α MAPK. This antibody does not cross-react with either JNK/SAPK or p42/44 MAPK or other isoforms of p38.
Species Reactivity:
Human, Mouse, Rat
Application
Dilutions
Western Blotting
1:2000
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.