品牌:CST,产品名称:p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 英文名称:p21 Waf1/Cip1 (12D1) Rabbit mAb Product name:p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 Isotype:Mouse IgG2a Sensitivity:Endogenous MW(kDa):21 Applications:WB , IP , IHC , IF , F , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human p21. Background: The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).
p21 Waf1/Cip1 (12D1) Rabbit mAb detects endogenous levels of total p21 protein. The antibody does not cross-react with other CDK inhibitors.
Species Reactivity:
Human, Monkey
Species predicted to react based on 100% sequence homology:
Dog
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Immunohistochemistry (Paraffin)
1:50
Immunofluorescence (Immunocytochemistry)
1:800
Flow Cytometry
1:200
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.