品牌:CST,产品名称:Oct-4A (C30A3) Rabbit mAb #2840 英文名称:Oct-4A (C30A3) Rabbit mAb Product name:Oct-4A (C30A3) Rabbit mAb #2840 Isotype:Mouse IgG1 Sensitivity:Endogenous MW(kDa):45 Applications:WB , IF , F , Purification: Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Oct-4A. Background: Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).
Oct-4A (C30A3) Rabbit mAb detects endogenous levels of total Oct-4A protein.
Species Reactivity:
Human, Mouse
Application
Dilutions
Western Blotting
1:1000
Immunofluorescence (Immunocytochemistry)
1:400
Flow Cytometry
1:400
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.