品牌:CST,产品名称:Oct-1 (D7B6) Rabbit mAb #8157 英文名称:Oct-1 (D7B6) Rabbit mAb Product name:Oct-1 (D7B6) Rabbit mAb #8157 Source:Rabbit Sensitivity:Endogenous MW(kDa):90 Applications:WB , IP , ChIP Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala367 of human Oct-1 protein. Background: Oct-1 (POU2F1) is a ubiquitously expressed, octamer-binding transcription factor containing a POU domain with a homeobox subdomain (1). Oct-1 has been shown to interact with several transcription factors in mediating specific gene expression, including SNAPc (2), OBF-1 (a B-cell transcriptional coactivator protein) (3), TFIIB (4), and TBP (TATA-box-binding protein) (5). Its POU DNA-binding domain allows Oct-1 the flexibility to facilitate the binding and recruitment of several tissue-specific cofactors to either positively or negatively regulate a variety of genes, exerting an important role in development (6).
Oct-1 (D7B6) Rabbit mAb recognizes endogenous levels of total Oct-1 protein.
Species Reactivity:
Human
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:100
Chromatin IP
1:50
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.