品牌:CST,产品名称:OB-Cadherin (16G5) Mouse mAb #13577
英文名称:OB-Cadherin (16G5) Mouse mAb
Product name:OB-Cadherin (16G5) Mouse mAb #13577
Isotype:Rabbit IgG
Sensitivity:Endogenous
MW(kDa):40, 44
Applications:WB , IF , F ,
Purification: Monoclonal antibody is produced by immunizing animals with recombinant protein containing the extracellular cadherin domain 1 of OB-cadherin fused to maltose binding protein.
Background: Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).OB-cadherin (CDH11) is highly expressed in osteoblastic cell lines (9). Its upregulation during differentiation in cells of the osteo-lineage and the chondro-lineage implies a specific role in bone cell differentiation and bone formation (9,10).
Entrez-Gene Id
1009
Swiss-Prot Acc.
P55287
Specificity / Sensitivity:
OB-Cadherin (16G5) Mouse mAb recognizes endogenous levels of total OB-cadherin protein.
Species Reactivity:
Human
Application |
Dilutions |
Western Blotting |
1:1000 |
Immunofluorescence (Immunocytochemistry) |
1:3200 |
Flow Cytometry |
1:3200 |
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.