品牌:CST,产品名称:N-WASP (30D10) Rabbit mAb #4848
英文名称:N-WASP (30D10) Rabbit mAb
Product name:N-WASP (30D10) Rabbit mAb #4848
Isotype:Rabbit IgG
Sensitivity:Endogenous
MW(kDa):60
Applications:WB , IP ,
Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding the sequence of human N-WASP.
Background: Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8).
Entrez-Gene Id
8976
Swiss-Prot Acc.
O00401
Specificity / Sensitivity:
N-WASP (30D10) Rabbit mAb recognizes endogenous levels of total N-WASP protein. The antibody does not cross-react wth the hematopietic protein, WASP.
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey, Bovine
Application |
Dilutions |
Western Blotting |
1:1000 |
Immunoprecipitation |
1:50 |
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.