品牌:CST,产品名称:Numb (C29G11) Rabbit mAb #2756 英文名称:Numb (C29G11) Rabbit mAb Product name:Numb (C29G11) Rabbit mAb #2756 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):78 Applications:WB , IP , IF , F , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala570 of human Numb protein. Background: Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9).
Numb (C29G11) Rabbit mAb detects endogenous levels of total Numb protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Immunofluorescence (Immunocytochemistry)
1:400
Flow Cytometry
1:400
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.