品牌:CST,产品名称:Myosin IIb (D8H8) XP Rabbit mAb #8824
英文名称:Myosin IIb (D8H8) XP® Rabbit mAb
Product name:Myosin IIb (D8H8) XP® Rabbit mAb #8824
Isotype:Rabbit IgG
Sensitivity:Endogenous
MW(kDa):230
Applications:WB , IF ,
Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human myosin IIb protein.
Background: Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).
Entrez-Gene Id
4628
Swiss-Prot Acc.
P35580
Specificity / Sensitivity:
Myosin IIb (D8H8) XP® Rabbit mAb recognizes endogenous levels of total myosin IIb protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Application |
Dilutions |
Western Blotting |
1:1000 |
Immunofluorescence (Immunocytochemistry) |
1:200 |
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.