品牌:CST,产品名称:MSH2 (D24B5) XP Rabbit mAb #2017 英文名称:MSH2 (D24B5) XP® Rabbit mAb Product name:MSH2 (D24B5) XP® Rabbit mAb #2017 Source:Rabbit Sensitivity:Endogenous MW(kDa):21 Applications:WB , IP , IHC , IF , F , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the amino terminus of human MSH2. Background: The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between non-identical DNA sequences and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins and the Rad50-Mre11-NBS1 complex (2).Mutations in MSH2 have been found in a large proportion of hereditary non-polyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations have also been associated with other sporadic tumors.
MSH2 (D24B5) XP® Rabbit mAb detects endogenous levels of total MSH2 protein.
Species Reactivity:
Human, Mouse
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:100
Immunohistochemistry (Paraffin)
1:200
Immunofluorescence (Immunocytochemistry)
1:100
Flow Cytometry
1:100
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.