品牌:CST,产品名称:NKX3.1 (D2Y1A) XP Rabbit mAb #83700 英文名称:NKX3.1 (D2Y1A) XP® Rabbit mAb Product name:NKX3.1 (D2Y1A) XP® Rabbit mAb #83700 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):30 Applications:WB , IF , ChIP Purification: Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human NKX3.1 protein. Background: NKX3.1 is a homeobox transcription factor that in mammals plays a defining role in embryonic prostate morphogenesis. The expression of mammalian NKX3.1 is androgen-dependent, restricted primarily to developing and mature prostate epithelium, and is frequently reduced or lost in prostate cancer (1-3). The human NKX3.1 gene is located on chromsome 8p21.2, within a region that shows loss of heterozygosity (LOH) in >50% of prostate cancer cases (2). Allelic loss at the NKX3.1 locus is also common in high grade Prostate Intraepithelial Neoplasia (PIN), thought to be a putative precursor lesion to invasive prostate adenocarcinomas, suggesting that LOH at the NKX3.1 locus is a critical early step in prostate cancer development (4). Notably, the remaining NKX3.1 allele is intact in the majority of LOH cases, leading to the suggestion that NKX3.1 functions as a haploinsufficient tumor suppressor (4-6). Due to its highly restricted expression in prostate epithelial cells, NKX3.1 has been suggested as a diagnostic marker of prostate carcinoma (7), and may have additional utility as a biomarker of metastatic lesions originating in the prostate (8).
NKX3.1 (D2Y1A) XP® Rabbit mAb recognizes endogenous levels of total NKX3.1 protein.
Species Reactivity:
Human
For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application
Dilutions
Western Blotting
1:1000
Immunofluorescence (Immunocytochemistry)
1:100
Chromatin IP
1:100
Chromatin IP-seq
1:100
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.