品牌:CST,产品名称:NF-κB1 p105/p50 (D4P4D) Rabbit mAb #13586
英文名称:NF-κB1 p105/p50 (D4P4D) Rabbit mAb
Product name:NF-κB1 p105/p50 (D4P4D) Rabbit mAb #13586
Isotype:Rabbit IgG
Sensitivity:Endogenous
MW(kDa):120, 52
Applications:WB , IP , IF , F , ChIP
Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile415 of mouse NF-κB1 p105/p50 protein.
Background: Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).Following IKK-mediated phosphorylation of p105 NF-κB at multiple sites (Ser921, 923, 927, and 932) on its carboxy terminus, SCF/β-TrCP-mediated processing produces the 50 kDa active form p50 (12,13).
Entrez-Gene Id
18033
Swiss-Prot Acc.
P25799
Specificity / Sensitivity:
NF-κB1 p105/p50 (D4P4D) Rabbit mAb recognizes endogenous levels of total NF-κB1 p105/p50 protein.
Species Reactivity:
Human, Mouse, Rat
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application |
Dilutions |
Western Blotting |
1:1000 |
Immunoprecipitation |
1:100 |
Immunofluorescence (Immunocytochemistry) |
1:200 |
Flow Cytometry |
1:100 |
Chromatin IP |
1:50 |
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.