品牌:CST,产品名称:Nanog (D73G4) XP Rabbit mAb (ChIP Formulated) #5232 英文名称:Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) Product name:Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) #5232 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):42 Applications:ChIP Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human nanog protein. Background: Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).
Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) detects endogenous levels of total nanog protein.
Species Reactivity:
Human
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application
Dilutions
Chromatin IP
1:50
Chromatin IP-seq
1:50
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.