品牌:CST,产品名称:LC3B (D11) XP Rabbit mAb #3868 英文名称:LC3B (D11) XP® Rabbit mAb Product name:LC3B (D11) XP® Rabbit mAb #3868 Source:Rabbit Sensitivity:Endogenous MW(kDa):14, 16 Applications:WB , IP , IHC , IF , F , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of LC3B. Background: Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).
LC3B (D11) XP® Rabbit mAb detects endogenous levels of total LC3B protein. Cross-reactivity may occur with other LC3 isoforms. Stronger reactivity is observed with the type II form of LC3B. Weaker reactivity is observed with rodent LC3B.
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology:
Monkey, Bovine, Pig
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Immunohistochemistry (Paraffin)
1:3200
Immunofluorescence (Immunocytochemistry)
1:200
Flow Cytometry
1:400
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.