品牌:CST,产品名称:IKKα (3G12) Mouse mAb (PE Conjugate) #54917 英文名称:IKKα (3G12) Mouse mAb (PE Conjugate) Product name:IKKα (3G12) Mouse mAb (PE Conjugate) #54917 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):110 cleaved. 270 full-length. Applications:F , Purification: Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to a fragment of human IKKα protein. Background: The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
IKKα (3G12) Mouse mAb (PE Conjugate) recognizes endogenous levels of total IKKα protein.
Species Reactivity:
Human, Monkey
Application
Dilutions
Flow Cytometry
1:50
Storage:
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Product Description:
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated IKKα (3G12) Mouse mAb #11930.