品牌:CST,产品名称:His-Tag (27E8) Mouse mAb #2366 英文名称:His-Tag (27E8) Mouse mAb Product name:His-Tag (27E8) Mouse mAb #2366 Isotype:Mouse IgG1 Sensitivity:Endogenous Applications:WB , IP , Purification: Monoclonal antibody is produced by immunizing animals with a 6xHis synthetic peptide. Background: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.A variety of plasmids contain DNA that encodes an amino-terminal tag cosisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography (1).As is the case with other protein tag systems (2), this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest (1).
Specificity / Sensitivity:
His-Tag (27E8) Mouse mAb detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Species Reactivity:
All Species Expected
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:200
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.