品牌:CST,产品名称:β-Arrestin 2 (C16D9) Rabbit mAb #3857 英文名称:β-Arrestin 2 (C16D9) Rabbit mAb Product name:β-Arrestin 2 (C16D9) Rabbit mAb #3857 Isotype:Rabbit Sensitivity:Endogenous MW(kDa):50 Reactivity:H,M,R,Mk Applications:WB , IHC , Purification: Monoclonal antibody is produced by immunizing animals with recombinant human β-arrestin 2. Background: Arrestin proteins function as negative regulators of G protein-coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as β-arrestin 1) and arrestin 3 (β-arrestin 2) are ubiquitously expressed and bind to most GPCRs (2). β-arrestins function as adaptor and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in Erk activation pathways (3,4). β-arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that β-arrestins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10).
β-Arrestin 2 (C16D9) Rabbit mAb detects endogenous levels of total β-arrestin 2 protein. It does not recognize transfected human β-arrestin 1.
Species Reactivity:
Human, Mouse, Rat, Monkey
Application
Dilutions
Western Blotting
1:1000
Immunohistochemistry (Paraffin)
1:50
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.