品牌:CST,产品名称:β-Actin (8H10D10) Mouse mAb (HRP Conjugate) #12262
英文名称:β-Actin (8H10D10) Mouse mAb (HRP Conjugate)
Product name:β-Actin (8H10D10) Mouse mAb (HRP Conjugate) #12262
Applications:WB ,
Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human β-actin protein.
Background: Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6).
Entrez-Gene Id
60
Swiss-Prot Acc.
P60709
Specificity / Sensitivity:
β-Actin (8H10D10) Mouse mAb (HRP Conjugate) recognizes endogenous levels of total β-actin protein.
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey, Dog
Application |
Dilutions |
Western Blotting |
1:1000 |
Storage:
Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Product Description:
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated β-Actin (8H10D10) Mouse mAb #3700.