TIM-3 (D5D5R™) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)
库存:
大量
供应商:
科敏商城
CAS号:
F ,
规格:
100 ul (50 tests)
品牌:CST,产品名称:TIM-3 (D5D5R™) XP Rabbit mAb (Alexa Fluor 488 Conjugate) #54669 英文名称:TIM-3 (D5D5R™) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) Product name:TIM-3 (D5D5R™) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #54669 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):45 Applications:F , Purification: Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human TIM-3 protein. Background: T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation (1-3 ). TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer (4,5), and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype (5). Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells (6). Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer (7). In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease (1). Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death (8).
TIM-3 (D5D5R™) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total TIM-3 protein.
Species Reactivity:
Human
Application
Dilutions
Flow Cytometry
1:50
Storage:
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Product Description:
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated TIM-3 (D5D5R™) XP® Rabbit mAb #45208.