品牌:CST,产品名称:TIM-3 (D3M9R) XP Rabbit mAb (Mouse Specific) #83882 英文名称:TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific) Product name:TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific) #83882 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):45-70 Applications:WB , IP , IHC , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro137 of mouse TIM-3 protein. Background: T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation (1-3 ). TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer (4,5), and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype (5). Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells (6). Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer (7). In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease (1). Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death (8).
TIM-3 (D3M9R) XP® Rabbit mAb (Mouse Specific) recognizes endogenous levels of total TIM-3 protein.
Species Reactivity:
Mouse
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:100
IHC-Leica® Bond™
1:800
Immunohistochemistry (Paraffin)
1:200
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.