品牌:CST,产品名称:TCPTP (TC45) (D7T7D) Rabbit mAb #58935 英文名称:TCPTP (TC45) (D7T7D) Rabbit mAb Product name:TCPTP (TC45) (D7T7D) Rabbit mAb #58935 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):48 Applications:WB , IP , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro382 of human TCPTP (TC45) protein. Background: T cell protein tyrosine phosphatase (TCPTP, PTPN2, PTN2) is a non-receptor protein tyrosine phosphatase (PTP) that regulates signal transduction pathways by catalyzing the dephosphorylation of tyrosine residues (1). Two described TCPTP splice variants include a 48 kDa isoform (TC48) that is targeted to secretory pathway organelles (e.g., endoplasmic reticulum) by a hydrophobic carboxy terminus, and a 45 kDa isoform (TC45) that actively shuttles between the nucleus and cytoplasm (2). TCPTP substrates include receptor and non-receptor tyrosine kinases, such as EGFR, JAK1/3, and Src-family kinases, as well as STAT3 and other nuclear substrates (3-6). Research studies show that the corresponding PTPN2 gene is deleted in a subset of human T-cell acute lymphoblastic leukemias. The loss of TCPTP has been suggested to promote tumor progression through enhanced JAK/STAT signaling (7,8).
TCPTP (TC45) (D7T7D) Rabbit mAb recognizes endogenous levels of total TCPTP (TC45) protein. This antibody does not recognize the 48 kDa (TC48) isoform.
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey
Species predicted to react based on 100% sequence homology:
Bovine, Dog, Pig
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.