品牌:CST,产品名称:Tau (D1M9X) XP Rabbit mAb #46687 英文名称:Tau (D1M9X) XP® Rabbit mAb Product name:Tau (D1M9X) XP® Rabbit mAb #46687 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):50-80 Applications:WB , IHC , IF , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp430 of human Tau protein. Background: Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).
Tau (D1M9X) XP® Rabbit mAb recognizes endogenous levels of total Tau protein.
Species Reactivity:
Human, Mouse, Rat
Application
Dilutions
Western Blotting
1:1000
Immunohistochemistry (Paraffin)
1:600
Immunofluorescence (Frozen)
1:100
Immunofluorescence (Immunocytochemistry)
1:100
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.