Survivin (71G4B7) Rabbit mAb (Alexa Fluor® 647 Conjugate)
库存:
大量
供应商:
科敏商城
CAS号:
F ,
规格:
100 ul (50 tests)
品牌:CST,产品名称:Survivin (71G4B7) Rabbit mAb (Alexa Fluor 647 Conjugate) #2866 英文名称:Survivin (71G4B7) Rabbit mAb (Alexa Fluor® 647 Conjugate) Product name:Survivin (71G4B7) Rabbit mAb (Alexa Fluor® 647 Conjugate) #2866 Applications:F , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding cysteine 60 of human survivin. The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm. Background: Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6).
Survivin (71G4E) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total survivin protein.
Species Reactivity:
Human, Mouse, Rat
Application
Dilutions
Flow Cytometry
1:50
Storage:
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Product Description:
Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody, #2808 reacts with survivin from human, mouse, and rat. CST expects that Survivin (71G4E) Rabbit mAb (Alexa Fluor® 647 Conjugate) will also recognize survivin in these species.