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SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Single Index Primers)
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科敏商城
ChIP
1 Kit (24 assays)
品牌:CST,产品名称:SimpleChIP ChIP-seq Multiplex Oligos for Illumina (Single Index Primers) #29580
英文名称:SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Single Index Primers)
Product name:SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Single Index Primers) #29580
Isotype:Rabbit IgG
Sensitivity:Endogenous
Applications:ChIP
Background: The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1,2). This assay can be used to identify multiple proteins associated with a specific region of the genome, or the opposite, to identify the many regions of the genome bound by a particular protein (3-6). It can be used to determine the specific order of recruitment of various proteins to a gene promoter or to "measure" the relative amount of a particular histone modification across an entire gene locus (3,4). In addition to histone proteins, the ChIP assay can be used to analyze binding of transcription factors and co-factors, DNA replication factors and DNA repair proteins. When performing the ChIP assay, cells or tissues are first fixed with formaldehyde, a reversible protein-DNA cross-linking agent that "preserves" the protein-DNA interactions occurring in the cell (1,2). Cells are lysed and chromatin is harvested and fragmented using either sonication or enzymatic digestion. The chromatin is then immunoprecipitated with antibodies specific to a particular protein or histone modification. Any DNA sequences that are associated with the protein or histone modification of interest will co-precipitate as part of the cross-linked chromatin complex and the relative amount of that DNA sequence will be enriched by the immunoselection process. After immunoprecipitation, the protein-DNA cross-links are reversed and the DNA is purified. Standard PCR or Quantitative Real-Time PCR can be used to measure the amount of enrichment of a particular DNA sequence by a protein-specific immunoprecipitation (1,2). Alternatively, the ChIP assay can be combined with genomic tiling micro-array (ChIP on chip) techniques, high throughput sequencing, or cloning strategies, all of which allow for genome-wide analysis of protein-DNA interactions and histone modifications (5-8).
This kit has been validated in combination with SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795 to generate qualified DNA libraries using 50 ng, 5 ng, or 0.5 ng ChIP DNA as starting materials. Libraries prepared from different starting amounts of ChIP DNA exhibit similiar Bioanalyzer® profiles, genome mapping rates, numbers of identified binding peaks, and signal-to-noise ratios across the whole genome.
All Species Expected
Product Includes | Volume (with Count) | Storage Temp |
---|---|---|
Adaptor for Illumina® | 1 x 240 µl | -20°C |
USER® Enzyme | 1 x 72 µl | -20°C |
Universal PCR Primer for Illumina® | 1 x 120 µl | -20°C |
Index 1 Primer for Illumina® | 1 x 10 µl | -20°C |
Index 2 Primer for Illumina® | 1 x 10 µl | -20°C |
Index 3 Primer for Illumina® | 1 x 10 µl | -20°C |
Index 4 Primer for Illumina® | 1 x 10 µl | -20°C |
Index 5 Primer for Illumina® | 1 x 10 µl | -20°C |
Index 6 Primer for Illumina® | 1 x 10 µl | -20°C |
Index 7 Primer for Illumina® | 1 x 10 µl | -20°C |
Index 8 Primer for Illumina® | 1 x 10 µl | -20°C |
Index 9 Primer for Illumina® | 1 x 10 µl | -20°C |
Index 10 Primer for Illumina® | 1 x 10 µl | -20°C |
Index 11 Primer for Illumina® | 1 x 10 µl | -20°C |
Index 12 Primer for Illumina® | 1 x 10 µl | -20°C |
Next generation sequencing (NG-seq) is a high throughput method that can be used downstream of chromatin immunoprecipitation (ChIP) assays to identify and quantify target DNA enrichment across the entire genome. SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Single Index Primers) contains adaptors and primers that are ideally suited for multiplex sample preparation for NG-seq on the Illumina® platform. This kit can be used to generate up to 12 distinct, barcoded ChIP-seq DNA libraries that can be combined into a single sequencing reaction.
This product provides enough reagents to support up to 24 DNA sequencing libraries, and must be used in combination with the SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795.
This product is compatible with SimpleChIP® Enzymatic ChIP Kit (Magnetic Beads) #9003, SimpleChIP® Plus Enzymatic ChIP Kit (Magnetic Beads) #9005, and SimpleChIP® Plus Sonication ChIP kit #56383. This product is not compatible with SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) #9002 and SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 because agarose beads are blocked with sonicated salmon sperm DNA, which will contaminate DNA library preps and NG-seq.
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