品牌:CST,产品名称:SHP-1 (C14H6) Rabbit mAb #3759 英文名称:SHP-1 (C14H6) Rabbit mAb Product name:SHP-1 (C14H6) Rabbit mAb #3759 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):68 Applications:WB , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro161 of human SHP-1. Background: SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain (1). SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth (2). Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation (3). Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R β chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation (4). Because SHP-1 downregulates various proliferation pathways, SHP-1 is considered a potential tumor suppressor and angiogenesis regulator (5,6).
SHP-1 (C14H6) Rabbit mAb detects endogenous level of total SHP-1 protein.
Species Reactivity:
Human, Mouse
Species predicted to react based on 100% sequence homology:
Rat
Application
Dilutions
Western Blotting
1:1000
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.