Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb
库存:
大量
供应商:
科敏商城
CAS号:
WB ,
规格:
20 ul (2 western blots)
品牌:CST,产品名称:Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb #9549 英文名称:Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb Product name:Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb #9549 Source:Rabbit Sensitivity:Endogenous MW(kDa):54 Applications:WB , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser380, Thr382 and Thr383 of human PTEN. Background: PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).
Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb detects endogenous levels of PTEN only when phosphorylated at Ser380, Thr382 and Thr383.
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology:
Chicken
Application
Dilutions
Western Blotting
1:1000
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.