品牌:CST,产品名称:Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb #14008
英文名称:Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb
Product name:Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb #14008
Source:Rabbit
Sensitivity:Endogenous
MW(kDa):155
Applications:WB , IP , F ,
Purification: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr783 of human PLCγ1 protein.
Background: Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6).
Entrez-Gene Id
5335
Swiss-Prot Acc.
P19174
Specificity / Sensitivity:
Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb recognizes endogenous levels of PLCγ1 protein only when phosphorylated at Tyr783.
Species Reactivity:
Human, Mouse
Species predicted to react based on 100% sequence homology:
Rat, Xenopus, Bovine, Dog
Application |
Dilutions |
Western Blotting |
1:1000 |
Immunoprecipitation |
1:50 |
Flow Cytometry |
1:400 |
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.