品牌:CST,产品名称:Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb #14717 英文名称:Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb Product name:Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb #14717 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):60 Reactivity:H Applications:WB , IP , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser93 of human Beclin-1 protein (Ser91 in mouse). Background: Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, research studies have shown it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). Beclin-1 is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Investigators have demonstrated that Beclin-1-/- mice die early in embryogenesis and Beclin-1-/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response, although responses to apoptosis appeared normal (7). Researchers have also found that overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4).To induce autophagy, AMP-activated protein kinase (AMPK) directly phosphorylates Beclin-1 at conserved Ser93 and Ser96 residues in human, which correspond to murine Ser91 and Ser94 (8).
Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb recognizes endogenous levels of Beclin-1 protein only when phosphorylated at Ser93. This antibody recognizes single phosphorylation of Ser93 to a greater extent than dual phosphorylation at Ser93 and Ser96. This antibody may also weakly detect an unidentified band at approximately 70 kDa.
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology:
Mouse, Rat, Bovine, Dog, Pig
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.