品牌:CST,产品名称:HA-Tag (6E2) Mouse mAb #2367 英文名称:HA-Tag (6E2) Mouse mAb Product name:HA-Tag (6E2) Mouse mAb #2367 Isotype:Mouse IgG1 Sensitivity:Transfected Only Applications:WB , IHC , IF , F , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) . Background: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been used extensively as a general epitope tag in expression vectors (1).
Specificity / Sensitivity:
HA-Tag (6E2) Mouse mAb detects recombinant proteins containing the HA epitope tag. The antibody recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Application
Dilutions
Western Blotting
1:1000
Immunohistochemistry (Paraffin)
1:1200
Immunofluorescence (Immunocytochemistry)
1:100
Flow Cytometry
1:50
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.