品牌:CST,产品名称:E2A (D2B1) Rabbit mAb #12258 英文名称:E2A (D2B1) Rabbit mAb Product name:E2A (D2B1) Rabbit mAb #12258 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):75 Applications:WB , IP , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly112 of human E2A protein. Background: E2A is a member of the E-protein family of transcription factors, a subclass of basic helix-loop-helix (bHLH) proteins that bind specifically to E-box consensus sequences (1,2). Alternative splicing generates two E2A isoforms (E47 and E12) that are actively involved in B cell lineage commitment, B cell maturation, IgK V-J rearrangement, peripheral B cell development, and tumor suppression (3). E2A acts in cis during G1 to promote immunoglobulin gene diversification (4). Research studies have shown that chromosomal translocations involving the E2A gene result in the expression of multiple fusion proteins and are associated with many cases of pediatric acute lymphoblastic leukemia (5).
E2A (D2B1) Rabbit mAb recognizes endogenous levels of total E2A protein.
Species Reactivity:
Human, Monkey
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.