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Di-Methyl-Histone H3 Antibody Sampler Kit
大量
科敏商城
1 Kit (6 x 20 ul)
品牌:CST,产品名称:Di-Methyl-Histone H3 Antibody Sampler Kit #9847
英文名称:Di-Methyl-Histone H3 Antibody Sampler Kit
Product name:Di-Methyl-Histone H3 Antibody Sampler Kit #9847
Purification: Polyclonal antibodies are produced by immunizing animals with synthetic di-methylated peptides corresponding to residues surrounding Lys 4 of human Histone H3. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic di-methylated peptides corresponding to residues surrounding Lys 9, 27, 36 and 79 of human Histone H3. Histone H3 (D1H2) XP Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human histone H3 protein.
Background: The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9).
All antibodies in the Di-Methyl-Histone H3 Antibody Sampler Kit recognize histone H3 only when modified at the indicated site.
Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
---|---|---|---|---|---|
Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb 9725 | 20 µl |
|
H M R Mk | 17 | Rabbit IgG |
Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb 4658 | 20 µl |
|
H M R Mk | 17 | Rabbit IgG |
Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb 2901 | 20 µl |
|
H M R Mk | 17 | Rabbit IgG |
Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb 9728 | 20 µl |
|
H M R Mk | 17 | Rabbit IgG |
Di-Methyl-Histone H3 (Lys79) (D15E8) XP® Rabbit mAb 5427 | 20 µl |
|
H M R Mk | 17 | Rabbit IgG |
Histone H3 (D1H2) XP® Rabbit mAb 4499 | 20 µl |
|
H M R Mk | 17 | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Goat |
The Di-Methyl-Histone H3 Antibody Sampler Kit provides a fast and economical means of evaluating methylation sites on histone H3. The kit contains enough primary and secondary antibodies to perform two western blots.
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