品牌:CST,产品名称:Cre Recombinase (D3U7F) Rabbit mAb #12830 英文名称:Cre Recombinase (D3U7F) Rabbit mAb Product name:Cre Recombinase (D3U7F) Rabbit mAb #12830 Isotype:Rabbit IgG Sensitivity:Transfected Only MW(kDa):37 Applications:WB , IF , F , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly103 of bacteriophage-P1 Cre recombinase protein. Background: Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34-base pair loxP sites. This reaction can be carried out in vitro, indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of in vitro and in vivo applications including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6).
Cre Recombinase (D3U7F) Rabbit mAb recognizes transfected levels of total Cre recombinase protein.
Species Reactivity:
All Species Expected
Application
Dilutions
Western Blotting
1:1000
Immunofluorescence (Immunocytochemistry)
1:300
Flow Cytometry
1:800
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.