品牌:CST,产品名称:CDK12 Antibody #11973 英文名称:CDK12 Antibody Product name:CDK12 Antibody #11973 Source:Rabbit Sensitivity:Endogenous MW(kDa):205 Applications:WB , IP , Purification: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val492 of human CDK12 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background: Cyclin-dependent kinase 12 (CDK12/CRKRS/CRK7) is composed of a central CTD kinase domain, several proline-rich regions, and several amino-terminal arginine/serine (RS) motifs common to splicing factors (1). CDK12 is ubiquitously expressed and forms a complex with cyclin K that regulates phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (1-4). CDK12 is important for expression of a subset of long genes with high numbers of exons including some regulators of the DNA damage response, such as breast and ovarian cancer type 1 susceptibility protein 1 (BRCA1) and ataxia telangiectasia and Rad3-related (ATR) (3). Depletion of CDK12 results in spontaneous DNA damage and increased sensitivity to DNA damage agents (3). Research studies have shown that CDK12 is recurrently mutated in high-grade ovarian cancer (5,6). In addition, high levels of CDK12 are required to maintain pluripotency of embryonic stem cells (7).