品牌:CST,产品名称:Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199
英文名称:Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb
Product name:Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199
Isotype:Rabbit IgG
Sensitivity:Endogenous
MW(kDa):20, 22
Reactivity:H
Applications:WB , IP ,
Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp297 of human caspase-1.
Background: Caspase-1, or interleukin-1ß converting enzyme (ICE/ICEα), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1ß and interferon-γ inducing factor (IL-18) into their mature forms (1,2). Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis (3). Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock (4,5). At least six caspase-1 isoforms have been identified, including caspase-1 α, β, γ, δ, ε and ζ (6). Most caspase-1 isoforms (α, β, γ and δ) produce products between 30-48 kDa and induce apoptosis upon over-expression. Caspase-1 ε typically contains only the p10 subunit, does not induce apoptosis and may act as a dominant negative. The widely expressed ζ isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the α isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the "inflammasome" that includes caspase-5, Pycard/Asc, and NALP1 (7).
Entrez-Gene Id
834
Swiss-Prot Acc.
P29466
Specificity / Sensitivity:
Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb detects endogenous levels of the p20 subunit of human caspase-1 only upon cleavage at Asp297.
Species Reactivity:
Human
Species predicted to react based on 100% sequence homology:
Monkey
Application |
Dilutions |
Western Blotting |
1:1000 |
Immunoprecipitation |
1:100 |
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.