品牌:CST,产品名称:Calbindin (D1I4Q) XP Rabbit mAb #13176 英文名称:Calbindin (D1I4Q) XP® Rabbit mAb Product name:Calbindin (D1I4Q) XP® Rabbit mAb #13176 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):28 Reactivity:H,M,R Applications:WB , IP , IHC , IF , Purification: Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human calbindin protein. Background: Calcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, parvalbumin and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2).
Calbindin (D1I4Q) XP® Rabbit mAb recognizes endogenous levels of total calbindin protein.
Species Reactivity:
Human, Mouse, Rat
Application
Dilutions
Western Blotting
1:1000
Immunoprecipitation
1:50
Immunohistochemistry (Paraffin)
1:800
Immunofluorescence (Frozen)
1:200
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.