品牌:CST,产品名称:Aprataxin (D5P3C) Rabbit mAb #52632 英文名称:Aprataxin (D5P3C) Rabbit mAb Product name:Aprataxin (D5P3C) Rabbit mAb #52632 Isotype:Rabbit IgG Sensitivity:Endogenous MW(kDa):40 Reactivity:H,M,R Applications:WB , Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe282 of human aprataxin protein. Background: Aprataxin is a DNA repair protein that resolves DNA lesions (DNA adenylates) caused by abortive ligations in single-strand break repair, double-strand break repair and base excision repair (1). Aprataxin is recruited to sites of DNA damage by PARP1 (2). In the mitochondria, aprataxin and tyrosyl-DNA-phosphodiesterase 1 (TDP1) are required for repair of single strand breaks caused primarily by reactive oxygen species (ROS) (3).The gene for aprataxin, APTX, is defective in the neurodegenerative disorder oculomotor apraxia type 1 (AOA1)(4). Researchers have shown that levels of aprataxin can predict patient response to irinotecan-based treatments in colorectal cancer (5).
Aprataxin (D5P3C) Rabbit mAb recognizes endogenous levels of total aprataxin protein.
Species Reactivity:
Human, Mouse, Rat
Application
Dilutions
Western Blotting
1:1000
Storage:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.