品牌:CST,产品名称:Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (HRP Conjugate) #14052
英文名称:Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (HRP Conjugate)
Product name:Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (HRP Conjugate) #14052
Applications:WB ,
Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 protein in which Lys9 is acetylated.
Background: Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
Entrez-Gene Id
8350
Swiss-Prot Acc.
P68431
Specificity / Sensitivity:
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (HRP Conjugate) detects endogenous levels of histone H3 protein only when acetylated on Lys9. This antibody does not cross-react with other acetylated histones.
Species Reactivity:
Human, Mouse, Rat, Monkey, Zebrafish
Species predicted to react based on 100% sequence homology:
S. cerevisiae
Application |
Dilutions |
Western Blotting |
1:1000 |
Storage:
Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Product Description:
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649.