- ￥6100
- GeneTex
- 美国
- GTX20304
- 2025年07月14日
- WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic phosphopeptide derived from human PKC θ around the phosphorylation site of Threonine 538.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX20304
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
PKC theta (phospho Thr538)
- 抗体英文名：
PKC theta (phospho Thr538) antibody
- 抗体名：
PKC theta (phospho Thr538) 抗体
- 规格：
100 μl
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Thr538) antibody (GTX20304) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
WB analysis of various samples using GTX20304 PKC theta (phospho Thr538) antibody.
Lane1 : MCF-7 whole cell lysate
Lane2 : MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate
Lane3 : MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate
Dilution : 1:500
WB analysis of various samples using GTX20304 PKC theta (phospho Thr538) antibody.
Lane1 : The Spleen tissue lysate of Rat
Lane2 : The Spleen tissue lysate of Mouse
Lane3 : L02 treated with LPS(100ng/ml) for 30 minutes whole cell lysate
Lane4 : HEK293T treated with Etoposide(20ng/ml) for 6 hours whole cell lysate
Lane5 : HEK293T treated with TNFα(20ng/ml)for 20 minutes whole cell lysate
Dilution : 1:500
Loading : 40ug
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Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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