This antibody was raised against a synthetic peptide corresponding to a sequence at the N-terminus of human CIAS1(12-31aa RYLEDLEDVDLKKFKMHLED), different from the related rat and mouse sequences by one amino acid.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse, Rat
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX00763
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IHC-P, FACS
浓度:
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
NLRP3
抗体英文名:
NLRP3 antibody
抗体名:
NLRP3 抗体
规格:
100 μg
IHC-P analysis of rat colon tissue section using GTX00763 NLRP3 antibody. Red : Primary antibody Blue : DAPI Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins Dilution : 1μg/ml
IHC-P analysis of rat brain tissue section using GTX00763 NLRP3 antibody. Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins Dilution : 1μg/ml
FACS analysis of U937 cells using GTX00763 NLRP3 antibody. Blue : Primary antibody Green : Isotype control Red : Blank control Antibody amount : 1μg/1x10⁶ cells
IHC-P analysis of rat spleen tissue section using GTX00763 NLRP3 antibody. Red : Primary antibody Blue : DAPI Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins Dilution : 1μg/ml
ICC/IF analysis of THP-1 cells using GTX00763 NLRP3 antibody. Antigen retrieval : Enzyme antigen retrieval was performed for 15 mins Dilution : 2μg/ml
IHC-P analysis of rat colon tissue section using GTX00763 NLRP3 antibody. Red : Primary antibody Blue : DAPI Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins Dilution : 1μg/ml
WB analysis of various samples using GTX00763 NLRP3 antibody. Lane 1 : HepG2 whole cell lysate Lane 2 : A549 whole cell lysate Lane 3 : U87 whole cell lysate Lane 4 : CEM whole cell lysate Dilution : 0.5 μg/mL Loading : 50μg
IHC-P analysis of human tonsil tissue using GTX00763 NLRP3 antibody. Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins. Dilution : 1μg/ml
FACS analysis of THP-1 cells using GTX00763 NLRP3 antibody. Blue : Primary antibody Green : Isotype control Red : Blank control Antibody amount : 1μg/1x10⁶ cells
ICC/IF analysis of THP-1 cells using GTX00763 NLRP3 antibody. Antigen retrieval : Enzyme antigen retrieval was performed for 15 mins Dilution : 2μg/ml
IHC-P analysis of rat kidney tissue section using GTX00763 NLRP3 antibody. Red : Primary antibody Blue : DAPI Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH 6) for 20 mins Dilution : 1μg/ml
Application Reference
Lou Q et al., Int J Nanomedicine 2023 (PMID:37192892)
Arab HH et al., Pharmaceuticals (Basel) 2022 (PMID:35890148)
Lo J et al., J Inflamm Res 2022 (PMID:36131784)
Arab HH et al., Pharmaceuticals (Basel) 2022 (PMID:36422532)
Erin Clough et al., J Neuroimmune Pharmacol 2021 (PMID:34599743)