- ￥1700 - 4000
- GeneTex
- 美国
- GTX133181
- 2025年07月15日
- WB
- Rabbit
- Human, Mouse, Rat, Drosophila
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding phospho Thr37/Thr46 of human 4E-BP1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Drosophila
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX133181
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB
- 浓度：
0.17 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
4E-BP1 (phospho Thr37/Thr46)
- 抗体英文名：
4E-BP1 (phospho Thr37/Thr46) antibody
- 抗体名：
4E-BP1 (phospho Thr37/Thr46) 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:1000.
Wild-type (WT) and 4E-BP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Cardile A et al., Int J Mol Sci 2023 (PMID:36674794)
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Expression of Recombinant Antibody Fusion Proteins in E. coli
Conjugates of antibodies or antibody fragments with foreign proteins have attracted interest because of their therapeutic potential as antitumor agents in vivo. Localization of the complex to the target site is dependent on the specificity
Expression and Isolation of Recombinant Antibody Fragments in E. coli
antibodies (MAbs), functional derivatives (antibody fragments) carrying antigen (Ag)-binding activities, and comprising at minimum the antibody variable heavy- and light-chain domains, can be readily produced in bacterial cells (1 ). The range of antibody
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